Industries & Nanozyme Application

download our brochure

industrial application

Dye & Textiles Industries

MICROBIAL NANOZYMETO EFFLUENT TREATMENT PLANT – BIOLOGICAL TREATMENT METHOD

The effluents generated from textile dyeing units create major environmental problems and issues both in public and textile units. Industrial wastewater treatment is one of the major problems in the present scenario. Generally, synthetic dyes can be classified as anionic (direct, acid and reactive dyes), cationic (basic) and non-ionic (disperse). Anionic dyes (direct, acid and reactive) represent 20–30% of commercial dyes used. Azo dyes are the largest chemical class of dyes with a great deal of structural and color variety used in industries representing up to 70% of the annual production. Synthetic dyes are released into the environment from textile industrial effluents.

DYE EFFLUENT AFTER NANOZYME BIOLOGICAL TREATMENT

Merits of using NANOZYME

Reduction of Usage of number of blowers and blower time so that it REDUCES THE MORE THAN 50 % OF ELECTRICITY USAGE OF ETP OPERATION.

LOW COST TREATMENT, ONE TIME RECOMMENDED DOSAGE of application further TRACE AMOUNT based on processing capacity.

Reduction of Up to 98% Reduction in BOD & 98% Reduction in COD load, TDS, Inorganic, organics and heavy metals it’s SAVE AND INCREASE THE LIFE OF MEMBRANE.

Reduction of sludge generation so that reduces the LABOUR COST by employing SLUDGE HANDLING.

Reduction in COLOR AND ODOR OF THE EFFLUENT. No messy cow dung handling, Improved Anaerobic digester efficiency.

More than 50 % REDUCTION OF USAGE OF CHEMICALS SO that TREATMENT COST WILL GET REDUCED.

The bacteria function as millions of tiny enzyme factories to produce the correct balance of degradative power.

Bacteria can produce the complete "team" of enzymes that are necessary to degrade and consume the organic materials present in their environment at any given time

Bacteria can adapt to a range of conditions and food supplies. They can change the type of enzymes that they produce if the food source changes. They can protect themselves from changes in environmental conditions by forming colonies, biofilms, or spores

Production of enzymes begins as soon as the bacteria begin to grow. The cells must obtain nutrients from their surroundings, so they secrete enzymes to degrade the available food.

The quantities of enzymes produced vary depending on the bacterial species and the culture conditions (e.g., nutrients, temperature, and pH) and growth rate. Hydrolytic enzymes such as proteases, amylases, and celluloses, etc. are produced in the range of milligrams per liter to grams per liter. Bacteria can grow very quickly, doubling their populations in as little as 20-40 minutes. In some applications, it is common to "boost" bacterial olonization.

NANOZYME Application Methods

Instead of direct application of first dose to aeration, we will keep it for mass activation (Incubation) with Jaggery and Nutrients in your site. This incubation of microbes will help to proliferate the microbial population and it also reduces the actual requirement of buying quantity of microbes.

Dosage Details

Effective temperature: Min. 20, - 60 °C

Effective pH Range : Min. 3 – 12

Number of Dosage: 10- 13 diluted NANOZYME- Its depends on the effluent characteristics.

NANOZYME -BET- ETP -7001

NANOZYME -BET- ETP -7002

NANOZYME -BET- ETP -7003

Recommended Dosage: NANOZYME – BET-ETP-7003- 10 Kilograms/100KLD

NUTRITION-BET- NUTRI 9101- 10 Kilograms/100 KLD

PAPER & PULP INDUSTRIES

PAPER & PULP INDUSTRIES - UPGRADATION OF BIOLOGICAL TREATMENT

Economic benefits of the pulp and paper industry have led it to be one of the most important industrial sections in the world. paper mills are categorized as a core sector industry and are the fifth largest contributor to industrial water pollution. The agro-based pulp and paper mills are highly water intensive, consuming 100–250 m3 fresh water/ton paper produced. These units correspondingly generate large quantities of wastewater, approximately 150–200 m3effluent/ton of paper produced. The problems associated with paper mill effluents are pH, colour, high levels of Biochemical Oxygen Demand (BOD), Chemical Oxygen Demand (COD), Suspended Solids (SS), Adsorbable Organic Halides (AOX) etc. Paper manufacturing process release chlorinated lignosulphonic acids, chlorinated resin acids, chlorinated phenols (trichlorophenol, trichloroguicol, tetrachloroguicol, dichlorophenol, dichloroguicol and pentachlorophenol), and chlorinated hydrocarbon in the effluent, are the major contaminants formed in the effluent of pulp and paper mill.

The raw wastewater consists pH of 6.8-7.1,Suspended Solids of 1160-1380mg/l, Total Dissolved Solids ranges from 1043-1293mg/l, BOD and COD varies 268 - 387 mg/l and 1110 –1272 mg/l respectively. After treatment pH varies 7.1 -7.3, Suspended Solids 322-505 mg/l, Total dissolved solids ranges from 807-984 mg/l , BOD and COD ranges from 176- 282 mg/l and 799-1002 mg/l, respectively

ROLE OF MICROBES IN THE DEGRADATION OF PAPER MILL EFFLUENT

An enzyme is a globular protein with an active site which bind to substrate molecules and helps to catalyse a reaction by holding molecules in the correct spatial conformation for the reaction to take place. Number of bacterial species has been assessed for their decolorization abilities and a few of them have also been used commercially. The dominant aboriginal microbes were found competent of reducing BOD up to 87.2%, COD up to 94.7% and lignin content up to 97%. It has been observed that although, numerous bacteria can decompose monomeric lignin substructure models, only a few strains are able to attack lignin derivatives obtained from different pulping processes. some of microbial enzyme was successfully used in a large-scale bio-bleaching that bleaches pulp optimally at pH 9 and 65°C temperature

The raw wastewater consists pH of 6.8-7.1,Suspended Solids of 1160-1380mg/l, Total Dissolved Solids ranges from 1043-1293mg/l, BOD and COD varies 268 - 387 mg/l and 1110 –1272 mg/l respectively. After treatment pH varies 7.1 -7.3, Suspended Solids 322-505 mg/l, Total dissolved solids ranges from 807-984 mg/l , BOD and COD ranges from 176- 282 mg/l and 799-1002 mg/l, respectively

  • NANOZYME effectively treats the suspended and floatable organic debris

  • Reduces more than 95% COD (Chemical Oxygen Demand),

  • Reduces more than 96 %BOD (Biological Oxygen Demand),

  • Reduces TSS (Total Suspended Solids) and 50% of TDS (Total dissolved solids)

  • LOW COST TREATMENT and Increases efficiency of the Effluent treatment plant and saves energy costs

  • Nanozyme is purely organic so, Non-corrosive, non-pathogenic and low quantities of use, making it safe and easy to handle and store.

  • Results meet criteria for new Pollution Control norms.

  • No design or process modifications required

HOW DO BACTERIA BREAK DOWN ANY MOLECULE?

Bacteria have the capability of producing many different types of enzymes that degrade a wide variety of organic materials such as fats, oils, cellulose, xylan, proteins starches and all chemicals.

  • The polymers that must be reacted with more than one type of enzyme in order to be efficiently degraded to their basic building blocks.

  • Bacteria can produce the complete "team" of enzymes that are necessary to degrade and consume the organic materials present in their environment at any given time.

  • Bacteria can adapt to a range of conditions and food supplies. They can change the type of enzymes that they produce if the food source changes.

  • They can protect themselves from changes in environmental conditions by forming colonies, biofilms, or spores.

Cellulose Degradation by bacteria

Cellulose is a structural polysaccharide that contains glucose. In order to access this glucose for catabolism, the cellulose must be cleaved by extracellular enzymes. These pieces are then transported into the cell for energy generation (catabolism) or production of biomass (anabolism). Some bacteria are important participants in the extracellular cleavage of cellulose.

Lignin degradation by bacteria

Lignin is the second most abundant renewable biopolymer in nature after cellulose, yet it is degraded by only a small number of microorganisms. Ligninolytic enzymes are involved in the degradation of the complex and recalcitrant polymer lignin. This group of enzymes are highly versatile in nature and they find application in a wide variety of industries. These aromatics can be released from the lignin sturucture by microbial enzymes such as peroxidases and oxidases. The enzymes utilize H2O2 and OH radicals to break the bonds in the lignin. Peroxidases are classified as oxido reductases which are used for catalyzing various oxidative reactions. They are isolated from various sources like plants, animals and microbes.

Aeration Tank -Activated Sludge Process

The secretions include slimes and gels, that may bond the bacteria together, and also enzymes. The enzymes break down large organic molecules into smaller monomers that are small enough to be ingested. The bacteria use the ingested molecules for the synthesis of new molecules, in the process of growth. When they have reached normal size, the bacterium divides into two, and the process is repeated. If nutrient molecules are not limiting, this results in exponential growth in the numbers of bacteria. The flocs are formed from aggregates of non-living organic polymers that are probably secreted by bacteria. This has the advantage that the enzymes that are secreted by the bacteria into the water, will tend to be confined in the vicinity of the substrate, thereby facilitating their digestion.

NANOZYME APPLICATION METHODS

Instead of direct application of first dose to aeration, we will keep it for mass activation (Incubation) with Nutrients in your site. This incubation of microbes will help to proliferate the microbial population and it also reduces the actual requirement of buying quantity of microbes.

1. Dosage Details: Depends on Effluent characteristics the dosage will vary. Recommended dosage is 8- 10 kgs/100 KLD of effluent. this recommended dosage is mixed with nutrients with 30 times of water its come 300 litres of microbial broth. keep it for 5 days incubation. After 5 days we can use it for dosage of 20- 30 times based on requirement. we will use the incubated microbial broth at different interval periods. Its depends on the effluent characteristics and degradation of pollutant load.

2. Effective temperature: Min. 20, - 60 °C

3. Effective pH Range : Min. 3 – 12

Recommended Dosage: NANOZYME – BET-ETP-7003- 10 Kilograms/100KLD

NUTRITION-BET- NUTRI 9101- 10 Kilograms/100 KLD

MICROBIAL NANOZYME TO TANNERY

MICROBIAL NANOZYME TO TANNERY - COMMON EFFLUENT TREATMENT PLANT

Among the industries tannery industries effluent is the most hazardous industrial pollutants due to its huge organic and inorganic load, which is highly toxic to human life and environment. One ton of skin generally leads to the production of 20 to 80 m3 of turbid and foul-smelling wastewater including chromium (100–400 mg/l), sulfide (200–800 mg/l), high levels of fat and other solid wastes, and notable pathogen contamination as well as pesticides added for skin conservation during transport. The quantity of effluent generated is about 30 L for every kilogram of hide or skin processed. The major components of the tannery effluent include sulfide, chromium, volatile organic compounds, large quantities of solid waste, suspended solids like animal hair and trimmings.. The combined effect of those pollutants (i.e. organic carbon, nutrients, chromium and other toxic substances) leads to drastic depletion of DO level in fresh water, death of fish population and excessive growth of water hyacinth or development of chromium toxicity.

Biological treatment involves stabilization of waste by decomposing them into harmless inorganic solids either by aerobic, anaerobic or combined process. Salinity of tannery wastewater makes it difficult to be treated by conventional biological treatment. Salt tolerant microbes can adapt to these saline conditions and degrade the organics in saline wastewater. Mechanism of Cr-tolerance or resistance of selected microbes is of particular importance in the bioremediation of contaminated tannery wastewater (Polisak et al. 2009). Biological treatment involves stabilization of waste by decomposing them into harmless inorganic solids either by aerobic, anaerobic or combined process.

SULFIDE REMOVAL

High sulfide concentration present in the wastewater may render aerobic biological treatment unsuitable. Sulfide removal is not an easy task and might require integration of different treatment units to be achieved. The low efficiency may be attributed to either the absence of sulfate oxidizing bacteria or deficiency of enough oxygen required for oxidation of sulfide into sulfate. To enhance removal of H2S from tannery effluent longer time is needed providing that enough oxygen is available or otherwise oxidation post treatment step should be taken. Sulfate-reducing bacteria and also some bacteria spp. reported capable of reducing Cr (VI) to Cr (III), thus are promising in bioremediation of chromium through bio-absorption and bioaccumulation.

ADVANTAGES OF USING NANOZYME

  • Reduction of Usage of number of blowers and blower time so that it REDUCES THE MORE THAN 30 % OF ELECTRICITY USAGE OF ETP OPERATION.

  • LOW COST TREATMENT, ONE TIME RECOMMENDED DOSAGE of application further TRACE AMOUNT based on processing capacity.

  • Reduction of Up to 90% Reduction in BOD & 90 % Reduction in COD load, TDS, Inorganic, organics and heavy metals it’s SAVE AND INCREASE THE LIFE OF MEMBRANE

  • Reduction of sludge generation so that reduces the LABOUR COST by employing SLUDGE HANDLING.

  • Reduction in COLOR AND ODOR OF THE EFFLUENT. No messy cow dung handling, Improved Anaerobic digester efficiency.

  • More than 50 % REDUCTION OF USAGE OF CHEMICALS SO that TREATMENT COST WILL GET REDUCED.

  • The bacteria function as millions of tiny enzyme factories to produce the correct balance of degradative power.

  • Bacteria can produce the complete "team" of enzymes that are necessary to degrade and consume the organic materials present in their environment at any given time.

  • Bacteria can adapt to a range of conditions and food supplies. They can change the type of enzymes that they produce if the food source changes. They can protect themselves from changes in environmental conditions by forming colonies, biofilms, or spores.

  • Production of enzymes begins as soon as the bacteria begin to grow. The cells must obtain nutrients from their surroundings, so they secrete enzymes to degrade the available food.

  • The quantities of enzymes produced vary depending on the bacterial species and the culture conditions (e.g., nutrients, temperature, and pH) and growth rate. Hydrolytic enzymes such as proteases, amylases, and celluloses, etc. are produced in the range of milligrams per liter to grams per liter. Bacteria can grow very quickly, doubling their populations in as little as 20-40 minutes. In some applications, it is common to "boost" bacterial colonization.

NANOZYME APPLICATION METHODS

Instead of direct application of first dose to aeration, we will keep it for mass activation (Incubation) with Nutrients in your site. This incubation of microbes will help to proliferate the microbial population and it also reduces the actual requirement of buying quantity of microbes.

Effluent Characteristics

S.No. Effluent Parameters Range
1. pH 8-8.5
2. COD 6000
3. BOD 1700-1800
4. TSS 500-600
5. TDS 23,000 ppm

Dosage Details

Effective temperature: Min. 20, - 60 °C

Effective pH Range : Min. 3 – 12

Number of Dosage: 10- 13 diluted NANOZYME- Its depends on the effluent characteristics.

NANOZYME -BET- ETP -7001

NANOZYME -BET- ETP -7002

NANOZYME -BET- ETP -7003

Recommended Dosage: NANOZYME – BET-ETP-7003- 10 Kilograms/100KLD

NUTRITION-BET- NUTRI 9101- 10 Kilograms/100 KLD

PHARMACEUTICALS INDUSTRIES

PHARMACEUTICALS INDUSTRIES

Distillery industries contributing the lion's share in the economic growth of the country. In the year 1999, there were 285 distilleries in India producing 2.7x109 l of alcohol and generating 4x1010 l of wastewater each year. There are 254distilleries in India producing 1000 million liters of alcohol and 3.5x 108 kiloliters of effluent each year. For the ethanol production Maximum Distillery industries present in the world are sugar base industries.. Typical BOD and COD values for a batch distillery spent wash are 35,000–50,000 and 80,000–100,000 mg/L, respectively, whereas for a continuous process, they are in the range of 60,000–100,000 and 160,000–200,000 mg/L, respectively. These industries required large amount of water for the production of alcohol and also generate a large amount of spent wash. Spent wash is the effluent generated during the alcohol production. Spent wash is dark brown, highly acidic, having very high chemical oxygen demand and biochemical oxygen demand. Distillery wastewaters contain phenolics compounds, mainly gallic acid, p-coumaric acid and gentisic acid, which imparthigh Antibacterial activity. Apart from this distillery wastewater also contains soluble proteins. Spent wash contains 2% melanoidin dark brown recalcitrant pigment, caramel, furfural and having high molecular weight nitrogenous brown polymer form by Maillard reaction between the amino acid and sugar.

Bio-methanation of distillery spent wash followed by aerobic treatment is the commonly used treatment to treat distillery. Aerobic treatment reduces the chemical oxygen demand (COD), and Biological oxygen demand up to 50 to 70 %, but till 100% colour, COD and BOD are not reduced. Implementation of physical, chemical and biological method to reduce the colour and chemical oxygen demand of spent wash generates significant amount of secondary sludge.

Anaerobic Treatment

ADVANTAGES OF ANAEROBIC TREATMENT

a. Low production of waste biological solids.

b. Low nutrient requirements.

c. Production of methane as an energy source to meet the steam requirement of distillery to the extent of 75-100%.

d. Very high loading rates can be achieved.

e. Active-anaerobic sludge can be preserved unfed for many months

Biocomposting

Biocomposting is an aerobic, thermophilic process resulting in a product rich in humus which is thus used as a fertilizer. This is a popular option adopted by several Indian distilleries attached to sugar mills with adequate land availability. The spent wash, either directly, or after biomethanation is sprayed in a controlled manner on sugarcane pressmud. The latter is the filter cake obtained during juice clarification in the manufacture of sugar.

ADVANTAGES OF USING NANOZYME

  • Reduction of Usage of number of blowers and blower time so that it REDUCES THE MORE THAN 30 % OF ELECTRICITY USAGE OF ETP OPERATION.

  • LOW COST TREATMENT, ONE TIME RECOMMENDED DOSAGE of application further TRACE AMOUNT based on processing capacity.

  • Reduction of Up to 90% Reduction in BOD & 90 % Reduction in COD load, TDS, Inorganic, organics and heavy metals it’s SAVE AND INCREASE THE LIFE OF MEMBRANE

  • Reduction of sludge generation so that reduces the LABOUR COST by employing SLUDGE HANDLING.

  • Reduction in COLOR AND ODOR OF THE EFFLUENT. No messy cow dung handling, Improved Anaerobic digester efficiency.

  • More than 50 % REDUCTION OF USAGE OF CHEMICALS SO that TREATMENT COST WILL GET REDUCED.

  • The bacteria function as millions of tiny enzyme factories to produce the correct balance of degradative power.

  • Bacteria can produce the complete "team" of enzymes that are necessary to degrade and consume the organic materials present in their environment at any given time.

  • Bacteria can adapt to a range of conditions and food supplies. They can change the type of enzymes that they produce if the food source changes. They can protect themselves from changes in environmental conditions by forming colonies, biofilms, or spores.

  • Production of enzymes begins as soon as the bacteria begin to grow. The cells must obtain nutrients from their surroundings, so they secrete enzymes to degrade the available food.

  • The quantities of enzymes produced vary depending on the bacterial species and the culture conditions (e.g., nutrients, temperature, and pH) and growth rate. Hydrolytic enzymes such as proteases, amylases, and celluloses, etc. are produced in the range of milligrams per liter to grams per liter. Bacteria can grow very quickly, doubling their populations in as little as 20-40 minutes. In some applications, it is common to "boost" bacterial colonization.

NANOZYME APPLICATION METHODS

Instead of direct application of first dose to aeration, we will keep it for mass activation (Incubation) with Nutrients in your site. This incubation of microbes will help to proliferate the microbial population and it also reduces the actual requirement of buying quantity of microbes.

Effluent Characteristics

S.No. Effluent Parameters Range
1. pH 8-8.5
2. COD 6000
3. BOD 1700-1800
4. TSS 500-600
5. TDS 23,000 ppm

Dosage Details

Effective temperature: Min. 20, - 60 °C

Effective pH Range : Min. 3 – 12

Number of Dosage: 10- 13 diluted NANOZYME- Its depends on the effluent characteristics.

NANOZYME -BET- ETP -7001

NANOZYME -BET- ETP -7002

NANOZYME -BET- ETP -7003

Recommended Dosage: NANOZYME – BET-ETP-7003- 10 Kilograms/100KLD

NUTRITION-BET- NUTRI 9101- 10 Kilograms/100 KLD

SEWAGE TREATMENT PLANT

MICROBIAL NANOZYME TO SEWAGE TREATMENT PLANT- UPGRADATION OF BIOLOGICAL TREATMENT SYSTEM

The term “sewage sludge” or “biosolids” represents the insoluble residue produced during wastewater treatment and subsequent sludge stabilization procedures, such as aerobic or anaerobic digestion. The term sewage refers to the wastewater produced by a community which may originate from three different sources: (a) domestic wastewater, (b) industrial wastewater, and (c) rain water. Depending on the degree of pollution and the sanitary requirements, all effluents can be either discharged straight into a stream or only after the appropriate treatment (mechanical, chemical, or biological). The sewage should be specially treated before disposal. “The term treatment means separation of solids and stabilisation of pollutants. In turn stabilisation means the degradation of organic matter until the point at which chemical or biological reactions stop. Treatment can also mean the removal of toxic or otherwise dangerous substances (for e.g. heavy metals or phosphorous) which are likely to distort sustainable biological cycles, even after stabilisation of the organic matter. Widely used terminology refers to three levels of wastewater treatment: primary, secondary, and tertiary (or advanced).

Primary (mechanical) treatment is designed to remove gross, suspended and floating solids from raw sewage. It includes screening to trap solid objects and sedimentation by gravity to remove suspended solids. This level is sometimes referred to as “mechanical treatment”, although chemicals are often used to accelerate the sedimentation process. Primary treatment can reduce the BOD of the incoming wastewater by 20-30% and the total suspended solids by some 50-60%. Primary treatment is usually the first stage of wastewater treatment.

Secondary (biological) treatment removes the dissolved organic matter that escapes primary treatment. This is achieved by microbes consuming the organic matter as food, and converting it to carbon dioxide, water, and energy for their own growth and reproduction. The biological process is then followed by additional settling tanks (“secondary sedimentation", see photo) to remove more of the suspended solids. About 85% of the suspended solids and BOD can be removed by a well running plant with secondary treatment. Secondary treatment technologies include the basic activated sludge process, the variants of pond and constructed wetland systems, trickling filters and other forms of treatment which use biological activity to break down organic matter.

Tertiary treatment is simply additional treatment beyond secondary! Tertiary treatment can remove more than 99 percent of all the impurities from sewage, producing an effluent of almost drinking-water quality. The related technology can be very expensive, requiring a high level of technical know-how and well trained treatment plant operators, a steady energy supply, and chemicals and specific equipment which may not be readily available. An example of a typical tertiary treatment process is the modification of a conventional secondary treatment plant to remove additional phosphorus and nitrogen.

Sewage composition

Sewage is composed of organic matter such as carbohydrates, fats, oil, grease and proteins mainly from domestic waste. It also contains dissolved inorganic matter such as nitrogen species and phosphorous species mainly from agricultural use. It is essential to remove the nutrients before they are released to the environment because it interferes natural habitats by altering the chemical composition such as pH or oxygen level both directly and indirectly.

Oxygen level

Oxygen level is an important factor to secondary and tertiary treatment processes. Secondary treatment, oxygen is required as a terminal electron acceptor in organic matter degradation. For example, nitrification by Nitrosomonas and Nitrobacter species requires dissolved oxygen to occur. Oxygen in secondary treatment is provided manually by pumping oxygen into the sewage continuously which occurs in an aeration tank. This process is also aerobic, but it depends on the diffusion of oxygen because most organic matter has been degraded by secondary treatment.

Nutrients availability

There are a lot of nutrients available in the sewage because of human waste and agricultural runoff. Bacteria can harvest the electron from organic matter and transfer it to a terminal electron acceptor which results in the breakdown of organic matter and energy conservation.

Microbial processes

After primary treatment, liquid and solid phases are physically separated. The liquid phase is treated with aeration to allow aerobic degradation of the nutrients. The two important microbial processes at this stage are nitrification and phosphorous removal. Nitrification occurs in two discrete steps. First of all, ammonium is oxidized to nitrite by some bacteria, and nitrite is further oxidized to nitrate by some bacteria. Phosphorous removal can occur biologically by the process of “enhanced biological phosphorous removal.” The process is demonstrated by the cell taking up phosphorous within their cell, and the biomass is filtered.

Degrading Bacteria can be classified into different types :

  • Aerobic types (which require oxygen to live)

  • Anaerobic (which can live without oxygen )

  • Facultative types can thrive under both aerobic and anaerobic conditions.

  • For waste digestion, we can identify several beneficial characteristics that we want our chosen bacteria to have. The "good" bacteria that we will choose must:

  • Consume (digest) a wide variety of organic material that are present in wastes.

  • Digest waste quickly and completely, without producing significant odors of noxious gas.

  • Not cause any disease in man or animals - they must be non-pathogenic.

  • Grow and reproduce quickly and readily in the environmental conditions found in waste disposal systems.

How bacteria and enzymes work to digest organic wastes?

This process is responsible for the digestion of organic waste.

BACTERIAL / ENZYME DIGESTION

Bacterial digestion is the process of bacteria, consuming organic matter. Enzymes act to break the organic matter into water soluble nutrients, which the bacteria digest. Using complex chemical reactions, the organic waste is metabolized down to water and carbon dioxide (the final metabolic waste products), providing the bacteria with energy for growth and reproduction. It may be simply shown by the following equation:

AEROBIC DIGESTION

Organic waste + water -----Enzyme----> Water soluble nutrients + oxygen ----Bacteria---> water + carbon dioxide

ANAEROBIC DIGESTION

Organic waste + Water ------Enzyme-------> Water Soluble Nutrients -----Bacteria---> Water + Carbon Dioxide

Organic waste is consumed by the bacteria, used as nutrients by the bacteria, and is no longer present to produce odors, sludge, pollution, or unsightly mess.

In the present proposal for the removal of pollutants from the sewage water by using NANOZYME (Effective Microbes (EM)- bacterial consortium manufactured in BIONICS ENVIRO TECH having potential to degrade all organic and inorganic compounds present in the sewage water.

ROLE OF MICROBES IN THE DEGRADATION OF SEWAGE EFFLUENT

An enzyme is a globular protein with an active site which bind to substrate molecules and helps to catalyse a reaction by holding molecules in the correct spatial conformation for the reaction to take place. The activities of the enzymes are determined by their 3-dimensional structure. Most enzymes are much larger than the substrates they act on and only a small portion of the enzyme around 3-4 aminoacids is directly involved in catalysis.

Our products are based on the latest advanced technology in biotechnology designed to meet the requirements of specific waste water treatment problems. Biological treatment method by microbes is the only method that can eliminate/degrade the waste problem. Specially selected and acclimated strains of microorganisms supplied in NANOZYME microbial enzyme products produce millions of times the levels of organic digesting catalysts produced by various type of microorganisms found naturally in different waste. Bacteria are living organisms that continually adapt and grow in the system. They consume the waste, chemicals, medicals.

Effective Microorganism (EM) is the consortia of valuable microorganisms which secretes organic acids and enzymes for utilization and degradation of anthropogenic compounds. Combination of various strains will successively promotes the growth of bacterial population, break down and digest the waste in both aerobic and anaerobic conditions to a far greater degree than single microbial strain. Bioremediation process involves detoxification and mineralization, where the waste is converted into inorganic compounds such as carbon dioxide, water and methane. When compounds are persistent in the environment, their biodegradation often proceeds through multiple steps utilizing different enzyme systems or different microbial populations.

BACTERIAL BIO REMEDIATION

Number of bacterial species has been assessed for their decolorization abilities and a few of them have also been used commercially. The dominant aboriginal microbes were found competent of reducing BOD up to 97.2%, COD up to 94.7%.

NANOZYME effectively treats the suspended and floatable organic debris

  • Reduces more than 95% COD (Chemical Oxygen Demand),

  • Reduces more than 96 %BOD (Biological Oxygen Demand),

  • Reduces TSS (Total Suspended Solids) and 50% of TDS (Total dissolved solids)

  • Cultures grow in either the aerobic and anaerobic conditions

  • LOW COST TREATMENT and Increases efficiency of the Effluent treatment plant and saves energy costs.

  • Nanozyme is purely organic so, Non-corrosive, non-pathogenic and low quantities of use, making it safe and easy to handle and store.

  • Results meet criteria for new Pollution Control norms

  • No design or process modifications required

HOW DO BACTERIA BREAK DOWN ANY MOLECULE?

Bacteria have the capability of producing many different types of enzymes that degrade a wide variety of organic materials such as fats, oils, cellulose, xylan, proteins starches and all chemicals.

  • The polymers that must be reacted with more than one type of enzyme in order to be efficiently degraded to their basic building blocks.

  • Bacteria can produce the complete "team" of enzymes that are necessary to degrade and consume the organic materials present in their environment at any given time.

  • Bacteria can adapt to a range of conditions and food supplies. They can change the type of enzymes that they produce if the food source changes

  • They can protect themselves from changes in environmental conditions by forming colonies, biofilms, or spores.

MERITS OF ADVANCED BIOLOGICAL TREATMENT TECHNOLOGIES

These include possible mineralization of the dyes to harmless inorganic compounds like carbon dioxide and water, and formation of a lesser quantity of relatively harmless sludge. The use of microorganisms for the biodegradation of dyes is an attractive alternative to the development of bioremediation processes for the treatment of textile wastewater. Biological methods are environmentally friendly, produce less sludge than physical and chemical systems, and are relatively inexpensive, as the running cost is low. Microbial discoloration can occur via biosorption, enzymatic degradation or a combination of both.

NANOZYME Application Methods

Instead of direct application of first dose to aeration, we will keep it for mass activation (Incubation) with Jaggery and Nutrients in your site. This incubation of microbes will help to proliferate the microbial population and it also reduces the actual requirement of buying quantity of microbes.

Dosage Details: Depends on Effluent characteristics the dosage will vary. Recommended dosage is 8- 10 kgs/100 KLD of effluent. This recommended dosage is mixed with nutrients and jaggery with 30 times of water its come 300 litres of microbial broth. keep it for 5 days incubation. After 5 days we can use it for dosage of 20- 30 times based on requirement. we will use the incubated microbial broth at different interval periods. Its depends on the effluent characteristics and degradation of pollutant load.

2. Effective temperature: Min. 20, - 60 °C

3. Effective pH Range : Min. 3 – 12

Recommended Dosage: NANOZYME – BET-STP-7003- 10 Kilograms/100KLD

NUTRITION-BET- NUTRI 9101- 10 Kilograms/100 KLD